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Notre expertise

Notre expérience, notre réseau

Notre expérience s’appuie sur plus de 20 ans de recherche dans le domaine de l'immunothérapie adoptive et de l'immunothérapie génique (avec le développement de lymphocytes génétiquement modifiés exprimant un gène suicide), en collaboration avec l'Etablissement Français du Sang Bourgogne/Franche-Comté et l'UMR Inserm 1098, ainsi que dans l'étude du carcinome hépatocellulaire, avec l'Institut de Recherche sur les Maladies Virales et Hépatiques et l'Institut Hospitalo-Universitaire de Strasbourg (Institut de Chirurgie Guidée par l'Image), conduisant à de nombreuses publications.

 

Références bibliographiques (sélection)

Humanisation of a claudin-1-specific monoclonal antibody for clinical prevention and cure of HCV infection without escape. Colpitts CC, Tawar RG, Mailly L, Thumann C, Heydmann L, Durand SC, Xiao F, Robinet E, Pessaux P, Zeisel MB, Baumert TF. Gut. 2018 Apr;67(4):736-745. doi: 10.1136/gutjnl-2016-312577. Epub 2017 Mar 30.

 

Non-invasive quantitative imaging of hepatocellular carcinoma growth in mice by micro-CT using liver-targeted iodinated nano-emulsions. Anton N, Parlog A, Bou About G, Attia MF, Wattenhofer-Donzé M, Jacobs H, Goncalves I, Robinet E, Sorg T, Vandamme TF. Sci Rep. 2017 Oct 24;7(1):13935. doi: 10.1038/s41598-017-14270-7.

 

Multimodal imaging of a humanized orthotopic model of hepatocellular carcinoma in immunodeficient mice. Wu T, Heuillard E, Lindner V, Bou About G, Ignat M, Dillenseger JP, Anton N, Dalimier E, Gossé F, Fouré G, Blindauer F, Giraudeau C, El-Saghire H, Bouhadjar M, Calligaro C, Sorg T, Choquet P, Vandamme T, Ferrand C, Marescaux J, Baumert TF, Diana M, Pessaux P, Robinet E. Sci Rep. 2016 Oct 14;6:35230. doi: 10.1038/srep35230.

 

Suicide gene-modified killer cells as an allogeneic alternative to autologous cytokine-induced killer cell immunotherapy of hepatocellular carcinoma. Wu T, Leboeuf C, Durand S, Su B, Deschamps M, Zhang X, Ferrand C, Pessaux P, Robinet E. Mol Med Rep. 2016 Mar;13(3):2645-54. doi: 10.3892/mmr.2016.4811. Epub 2016 Jan 27.

 

Use of a Closed Culture System to Improve the Safety of Lentiviral Vector Production. Wu T, Bour G, Durand S, Lindner V, Gossé F, Zona L, Certoux JM, Diana M, Baumert TF, Marescaux J, Mutter D, Pessaux P, Robinet E. Hum Gene Ther Methods. 2015 Dec;26(6):197-210. doi: 10.1089/hgtb.2015.080. Epub 2015 Nov 10.

 

Editorial: Improving the safety of cell therapy products by suicide gene transfer. Bole-Richard E, Deschamps M, Ferrand C, Robinet E. Front Pharmacol. 2015 Aug 25;6:174. doi: 10.3389/fphar.2015.00174. eCollection 2015. No abstract available.

 

Clearance of persistent hepatitis C virus infection in humanized mice using a claudin-1-targeting monoclonal antibody. Mailly L, Xiao F, Lupberger J, Wilson GK, Aubert P, Duong FHT, Calabrese D, Leboeuf C, Fofana I, Thumann C, Bandiera S, Lütgehetmann M, Volz T, Davis C, Harris HJ, Mee CJ, Girardi E, Chane-Woon-Ming B, Ericsson M, Fletcher N, Bartenschlager R, Pessaux P, Vercauteren K, Meuleman P, Villa P, Kaderali L, Pfeffer S, Heim MH, Neunlist M, Zeisel MB, Dandri M, McKeating JA, Robinet E, Baumert TF. Nat Biotechnol. 2015 May;33(5):549-554. doi: 10.1038/nbt.3179. Epub 2015 Mar 23.

 

Prevention of hepatitis C virus infection by adoptive allogeneic immunotherapy using suicide gene-modified lymphocytes: an in vitro proof-of-concept. Leboeuf C, Roser-Schilder J, Lambotin M, Durand S, Wu T, Fauvelle C, Su B, Bôle-Richard E, Deschamps M, Ferrand C, Tiberghien P, Pessaux P, Baumert TF, Robinet E. Gene Ther. 2015 Feb;22(2):172-80. doi: 10.1038/gt.2014.99. Epub 2014 Nov 13. PubMed PMID: 25394253.


In vivo proof of concept of adoptive immunotherapy for hepatocellular carcinoma using allogeneic suicide gene-modified killer cells. Leboeuf C, Mailly L, Wu T, Bour G, Durand S, Brignon N, Ferrand C, Borg C, Tiberghien P, Thimme R, Pessaux P, Marescaux J, Baumert TF, Robinet E. Mol Ther. 2014 Mar;22(3):634-644. doi: 10.1038/mt.2013.277. Epub 2013 Dec 6. PubMed PMID: 24445938; PubMed Central PMCID: PMC3944343.


Genetically engineered T-cells expressing a ganciclovir-sensitive HSV-tk suicide gene for the prevention of GvHD. Mailly L, Leboeuf C, Tiberghien P, Baumert T, Robinet E. Curr Opin Investig Drugs. 2010 May;11(5):559-70. Review. PubMed PMID: 20419602.


Early immune response against retrovirally transduced herpes simplex virus thymidine kinase-expressing gene-modified T cells coinfused with a T cell-depleted marrow graft: an altered immune response? Mercier-Letondal P, Deschamps M, Sauce D, Certoux JM, Milpied N, Lioure B, Cahn JY, Deconinck E, Ferrand C, Tiberghien P, Robinet E. Hum Gene Ther. 2008 Sep;19(9):937-50. PubMed PMID: 18810797.


Abnormal expression of only the CD34 part of a transgenic CD34/herpes simplex virus-thymidine kinase fusion protein is associated with ganciclovir resistance. Bennour E, Ferrand C, Rémy-Martin JP, Certoux JM, Gorke S, Qasim W, Gaspar HB, Baumert T, Duperrier A, Deschamps M, Fehse B, Tiberghien P, Robinet E. Hum Gene Ther. 2008 Jul;19(7):699-709. doi: 10.1089/hum.2007.060. PubMed PMID: 18557699.


Alloreactivity of ex vivo-expanded T cells is correlated with expansion and CD4/CD8 ratio. Mercier-Letondal P, Montcuquet N, Sauce D, Certoux JM, Jeanningros S, Ferrand C, Bonyhadi M, Tiberghien P, Robinet E. Cytotherapy. 2008;10(3):275-88. doi: 10.1080/14653240801927032. PubMed PMID: 18418773.


Regulatory T-cell expansion and function do not account for the impaired alloreactivity of ex vivo-expanded T cells. Montcuquet N, Mercier-Letondal P, Perruche S, Duperrier A, Couturier M, Bouchekioua A, Bonyhadi M, Ferrand C, Tiberghien P, Robinet E. Immunology. 2008 Nov;125(3):320-30. doi: 10.1111/j.1365-2567.2008.02843.x. Epub 2008 Apr 26. PubMed PMID: 18445006; PubMed Central PMCID: PMC2669136.


Transcriptome of retrovirally transduced CD8+ lymphocytes: influence of cell activation, transgene integration, and selection process. Deschamps M, Robinet E, Certoux JM, Mercier P, Sauce D, De Vos J, Montcuquet N, Bonyhadi M, Rème T, Tiberghien P, Ferrand C. Mol Immunol. 2008 Feb;45(4):1112-25. Epub 2007 Sep 10. PubMed PMID: 17825913.


Deletions within the HSV-tk transgene in long-lasting circulating gene-modified T cells infused with a
hematopoietic graft.
Deschamps M, Mercier-Lethondal P, Certoux JM, Henry C, Lioure B, Pagneux C, Cahn JY, Deconinck E, Robinet E, Tiberghien P, Ferrand C. Blood. 2007 Dec 1;110(12):3842-52. Epub 2007 Aug 23. PubMed PMID: 17717134.


Improving the ex vivo retroviral-mediated suicide-gene transfer process in T lymphocytes to preserve immune function. Robinet E, Fehse B, Ebeling S, Sauce D, Ferrand C, Tiberghien P. Cytotherapy. 2005;7(2):150-7. Review. PubMed PMID: 16040394.


Preferential retroviral-mediated transduction of EBV- and CMV-specific T cells after polyclonal T-cell activation. Sauce D, Mercier P, Battini JL, Ferrand C, Certoux JM, Manel N, Taylor N, Duperrier A, Hervé P, Tiberghien P, Robinet E. Gene Ther. 2004 Jun;11(12):1019-22. PubMed PMID: 15103318.


Retrovirus-mediated gene transfer in human primary T lymphocytes induces an activation- and transduction/selection-dependent TCR-B variable chain repertoire skewing of gene-modified cells. Coito S, Sauce D, Duperrier A, Certoux JM, Bonyhadi M, Collette A, Kuehlcke K, Hervé P, Tiberghien P, Robinet E, Ferrand C. Stem Cells Dev. 2004 Feb;13(1):71-81. PubMed PMID: 15068695.


Retrovirus-mediated gene transfer in polyclonal T cells results in lower apoptosis and enhanced ex vivo cell expansion of CMV-reactive CD8 T cells as compared with EBV-reactive CD8 T cells. Sauce D, Rufer N, Mercier P, Bodinier M, Rémy-Martin JP, Duperrier A, Ferrand C, Hervé P, Romero P, Lang F, Tiberghien P, Robinet E. Blood. 2003 Aug 15;102(4):1241-8. Epub 2003 May 1. PubMed PMID: 12730101.


Influence of ex vivo expansion and retrovirus-mediated gene transfer on primary T lymphocyte phenotype and functions. Sauce D, Tonnelier N, Duperrier A, Petracca B, de Carvalho Bittencourt M, Saadi M, Saas P, Ferrand C, Herve P, Tiberghien P, Robinet E. J Hematother Stem Cell Res. 2002 Dec;11(6):929-40. PubMed PMID: 12590708.


Retrovirus-mediated gene transfer in primary T lymphocytes impairs their anti-Epstein-Barr virus potential through both culture-dependent and selection process-dependent mechanisms. Sauce D, Bodinier M, Garin M, Petracca B, Tonnelier N, Duperrier A, Melo JV, Apperley JF, Ferrand C, Hervé P, Lang F, Tiberghien P, Robinet E. Blood. 2002 Feb 15;99(4):1165-73. PubMed PMID: 11830462.


Administration of herpes simplex-thymidine kinase-expressing donor T cells with a T-cell-depleted allogeneic marrow graft. Tiberghien P, Ferrand C, Lioure B, Milpied N, Angonin R, Deconinck E, Certoux JM, Robinet E, Saas P, Petracca B, Juttner C, Reynolds CW, Longo DL, Hervé P, Cahn JY. Blood. 2001 Jan 1;97(1):63-72. PubMed PMID: 11133743.


Retrovirus-mediated gene transfer in primary T lymphocytes: influence of the transduction/selection process and of ex vivo expansion on the T cell receptor beta chain hypervariable region repertoire. Ferrand C, Robinet E, Contassot E, Certoux JM, Lim A, Hervé P, Tiberghien P. Hum Gene Ther. 2000 May 20;11(8):1151-64. PubMed PMID: 10834617.


Differential effects of cyclosporin A on the alloreactivity of fresh and ex vivo-expanded T lymphocytes. Contassot E, Robinet E, Angonin R, Laithier V, Bittencourt M, Pavy JJ, Cahn JY, Hervé P, Tiberghien P. Bone Marrow Transplant. 1998 Dec;22(11):1097-102. PubMed PMID: 9877273.


In vivo alloreactive potential of ex vivo-expanded primary T lymphocytes. Contassot E, Murphy W, Angonin R, Pavy JJ, Bittencourt MC, Robinet E, Reynolds CW, Cahn JY, Hervé P, Tiberghien P. Transplantation. 1998 May 27;65(10):1365-70. PubMed PMID: 9625020.


A closed culture system for the ex vivo transduction and expansion of human T lymphocytes. Robinet E, Certoux JM, Ferrand C, Maples P, Hardwick A, Cahn JY, Reynolds CW, Jacob W, Hervé P, Tiberghien P. J Hematother. 1998 Jun;7(3):205-15. PubMed PMID: 9621254.